There are two key components of Evonetix’s approach:
A platform that is highly scalable, but still permits exquisite control at each site of synthesis; and
The ability to assemble high-fidelity DNA molecules using a process of error detection throughout assembly.
In addition, our approach will permit the synthesis of ‘difficult’ sequences, such as those with a high GC content or with repeats.
Silicon arrays for DNA synthesis
Our technology is based upon a novel silicon array, manufactured with semiconductor microfabrication techniques and capable of independent control of 10,000 miniaturised reaction sites, allowing massive parallelism in the DNA synthesis process and therefore very high throughput.
Synthesis of oligonucleotides
Our platform is compatible with conventional chemical and enzymatic DNA synthesis. With exquisite control at each of 10,000 sites of synthesis, we will build any required oligo at any of the sites, all of which are under parallel flow.
Assembly of high-fidelity DNA
Following synthesis, oligonucleotides are selectively released and annealed in a sequential building process during which errors are removed.
The key advantages of our technology:
Massive parallelism leading to high throughput
Controlled assembly process resulting in improved fidelity at long sequence lengths
The ability to synthesise challenging sequences